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KMID : 0380020060210020096
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 2 p.96 ~ p.102
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Optimization of Submerged Cultivation of Hericium erinaceum
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Jeong Jae-Hyun
Lee Keun-Eok
Lee Shin-Young
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Abstract
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Recently, it has been known that Hericium erinaceum is a one of the very useful functional materials with great attention in mushroom processing industry. In present study, a liquid culture which was not studied systematically until now, was conducted as a method of cultivation for H. erinaceum, and also examined the characteristics of the liquid culture and conditions of process optimization. A good basal medium was selected through the cultivation of 16 species mushroom media and the optimum condition for medium and cultivation were chosen by response surface method. From these results, the optimum condition of medium for mushroom was 3% glucose, 0.2% yeast extract/peptone (1 : 1) and 0.1% KH2PO4/ MgSO4 (1 : 1) and also the optimal culture condition was obtained at inoculum of 13.42%, temperature of 22.3℃ and pH of 5.7. The mycelial dry weight of 9 g/l was obtained under these conditions and this amount was about 1.7 times higher than that which were cultivated in basal medium for 8 days.
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KEYWORD
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Hericium erinaceum, mycelium, submerged culture, response surface analysis, optimization
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